Detection of Herpes Simplex Virus (Hsv-1 01:13-18

Background: Recent studies have suggested the role of herpes viruses in the etiopathogenesis of periodontal disease. Very few studies have explored the role of herpes virus in periodontal disease. The occurrence of herpes viruses may also vary depending on the race of the population studied. Herpes Simplex Virus (HSV 1&2) is frequently detected in gingival crevicular fluid of periodontal pockets, but only a few studies have been carried out on gingival tissue to detect the presence of herpes virus. Thus, the aim of the present study is to detect HSV 1&2 in gingival tissue of chronic periodontitis patients and compare their result with those having clinically healthy gingiva. Method: Gingival tissue samples were collected from 10 subjects with clinically healthy gingiva and 10 subjects with chronic periodontitis. Clinically healthy gingival specimens were obtained from the patients undergoing crown lengthening procedures. Gingival tissue samples for chronic periodontitis subjects were obtained from patients undergoing periodontal surgery. HSV 1&2 were detected using multiplex polymerase chain reaction technique. The data collected was analysed statistically using descriptive analysis (SPSS Version 20). Result: Chronic periodontitis patients revealed presence of HSV-1 in 8 samples (80%) and HSV-2 in 2 samples (20%). Conclusion: This study demonstrated that human viruses may occur in periodontitis lesions with relatively high prevalence.